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Additional info for An introduction to permaculture sheet mulching. Experimental strategies and techniques for the pacific northwest coast

Example text

1). During transcription, the coding strand of DNA serves as a template for synthesis of an RNA molecule. The sequence of the RNA molecule is determined by complementary base pairing so that the RNA is a complementary transcript (copy) of the coding strand of DNA. Transcription requires four ribonucleoside 5 -triphosphates: ATP, GTP, CTP, and UTP (recall that uracil substitutes for thymine in RNA). A sugar–phosphate bond is formed between the 3 -OH group of one nucleotide and the 5 -triphosphate of a second nucleotide by the enzyme RNA polymerase.

Studies on the chemical nature of the substance inducing transformation of pneumococcal types. J. Exp. Med. 79: 137–158. Baker, T. , and S. P. Bell (1998). Polymerases and the replisome: machines within machines. Cell 92: 295–305. , D. D. Dubey, and J. A. Huberman (2000). Heterogeneity of eukaryotic replicons, replicon clusters and replication foci. Chromosoma 108: 471–484. Bogan, J. , D. A. Natale, and M. L. DePamphilis (2000). Initiation of eukaryotic DNA replication: conservative or liberal?

The sliding-clamp proteins (proliferating cell nuclear antigen, or PCNA) in eukaryotes bind adjacent to the clamp-loading proteins, forming a ring around the template DNA. The clamp proteins then load the DNA polymerase onto the DNA at the primer–template junction. The ring formed by the sliding clamp maintains the association of the polymerase with its template as replication progresses, allowing the uninterrupted synthesis of long DNA molecules. Helicases unwind the template DNA ahead of the replication fork.

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An introduction to permaculture sheet mulching. Experimental strategies and techniques for the pacific northwest coast


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